Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody (ab51107)

Overview

  • Product nameAnti-Estrogen Receptor alpha (phospho S104 + S106) antibody
    See all Estrogen Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor alpha (phospho S104 + S106)
  • Specificityab51107 detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at serine 104/106.
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic phospho-peptide derived from human Estrogen Receptor alpha around the phosphorylation site of serine 104/106 (V-SP-P-SP-P).

  • Positive control
    • IHC-P: Human breast carcinoma tissue; WB: Estradiol- treated MCF7 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab51107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
ELISA
  • Application notesELISA: 1/10000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight (non-phosphorilated protein): 65 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      modifications
      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).
    • Cellular localizationNucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody images

    • All lanes : Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody (ab51107)

      Lane 1 : Untreated MCF7 cell lysate.
      Lane 2 : Estradiol-treated MCF7 cell lysate.


      Predicted band size : 65 kDa
    • ab51107 (1/100) staining Estrogen Receptor alpha (phospho S104 + S106) in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Right panel represents a negative control experiment done in the presence of the immunizing (blocking) phospho-peptide.

    References for Anti-Estrogen Receptor alpha (phospho S104 + S106) antibody (ab51107)

    ab51107 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"