Anti-Estrogen Receptor alpha (phospho S118) antibody (ab31477)

Overview

  • Product name
    Anti-Estrogen Receptor alpha (phospho S118) antibody
    See all Estrogen Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor alpha (phospho S118)
  • Specificity
    ab31477 detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at Serine 118.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) - derived from around phosphorylation site at Serine118.

  • Positive control
    • MCF7 cell extracts, breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab31477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • Function
    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    modifications
    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • Cellular localization
    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

Anti-Estrogen Receptor alpha (phospho S118) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser118) Antibody (ab31477) . The picture on the right is treated with the synthesized peptide.
  • All lanes : Anti-Estrogen Receptor alpha (phospho S118) antibody (ab31477) at 1/1000 dilution

    Lane 1 : MCF7 cell extract -treated with E2
    Lane 2 : MCF7 cell extract -untreated


    Predicted band size : 68 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-Estrogen Receptor alpha (phospho S118) antibody (ab31477)

This product has been referenced in:
  • Monsivais D  et al. Uterine ALK3 is essential during the window of implantation. Proc Natl Acad Sci U S A 113:E387-95 (2016). IHC-P ; Mouse . Read more (PubMed: 26721398) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 and 293)
Loading amount
1e+006 cells
Specification
MCF7 and 293
Treatment
293 not treated, MCF7 ss and stimulated with E2
Gel Running Conditions
Reduced Denaturing (4-15% gradient gel)
Blocking step
Odyssey blocking buffer as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jul 26 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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