Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478)

Overview

  • Product name
    Anti-Estrogen Receptor alpha (phospho S167) antibody
    See all Estrogen Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor alpha (phospho S167)
  • Specificity
    ab31478 detects endogenous levels of estrogen receptor alpha only when phosphorylated at Serine 167.
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Serine 167

Properties

Applications

Our Abpromise guarantee covers the use of ab31478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ELISA
  • Application notes
    ELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
    • Sequence similarities
      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      modifications
      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).
    • Cellular localization
      Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using ab31478 at 1/50. Left: Untreated; Right: Treated with synthesized phosphopeptide.
    • All lanes : Anti-Estrogen Receptor alpha (phospho S167) antibody (ab31478) at 1/500 dilution

      Lane 1 : HeLa cells treated with EGF
      Lane 2 : Untreated HeLa cell lysate


      Predicted band size : 68 kDa
    • ab31478 staining human non-small cell lung cancer tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in 10 mM sodium citrate (pH 6) prior to blocking with a commercially available blocking reagent for 20 minutes at 22°C.  The primary antibody was diluted 1/200 and incubated with the sample for 24 hours at 4°C.  A HRP conjugated antibody was used as the secondary.

      See Abreview

    References

    This product has been referenced in:
    • Lucchetti C  et al. The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor a in breast cancer. PLoS One 8:e55355 (2013). Read more (PubMed: 23390529) »
    • Zhao G  et al. MicroRNA-34a suppresses cell proliferation by targeting LMTK3 in human breast cancer mcf-7 cell line. DNA Cell Biol 32:699-707 (2013). WB . Read more (PubMed: 24050776) »

    See all 5 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Non-small cell lung cancer)
    Specification
    Non-small cell lung cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate, pH 6
    Permeabilization
    No
    Blocking step
    (agent) for 20 minute(s) · Concentration: 0µg/mL · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 06 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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