Anti-Estrogen Receptor alpha (phospho S305) antibody (ab61049)

Overview

• Product name
  Anti-Estrogen Receptor alpha (phospho S305) antibody
  See all Estrogen Receptor alpha primary antibodies
• Description
  Rabbit polyclonal to Estrogen Receptor alpha (phospho S305)
• Specificity
  Detects endogenous levels of Estrogen Receptor alpha only when phosphorylated at serine 305.
• Tested applications
  Suitable for: ELISA, ICC/IFmore details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic phosphopeptide derived from human Estrogen Receptor alpha around the phosphorylation site of serine 305 (K-N-S^P-L-A).

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • Nuclear Hormone Receptors
  • Estrogen

  • Neuroscience
  • Endocrine system
  • Gonadotrophic axis

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Estrogen

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • Nuclear hormone receptors
  • Estrogen

Associated products

• Assay kits
  • Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Target

• Function
  Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
• Sequence similarities
  Belongs to the nuclear hormone receptor family. NR3 subfamily.
  Contains 1 nuclear receptor DNA-binding domain.
• Domain
  Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
• Post-translational
  modifications
  Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
  Glycosylated; contains N-acetylglucosamine, probably O-linked.
  Ubiquitinated. Deubiquitinated by OTUB1.
  Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
  Palmitoylated (isoform 3). Not biotinylated (isoform 3).
• Cellular localization
  Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.

• Target information above from: UniProt accession P03372 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 2099 Human
  • Entrez Gene: 13982 Mouse
  • Omim: 133430 Human
  • SwissProt: P03372 Human
  • SwissProt: P19785 Mouse
  • Unigene: 208124 Human
  • Unigene: 463262 Mouse
  • Unigene: 9213 Mouse
• Alternative names
  • DKFZp686N23123 antibody
  • ER alpha antibody
  • ER antibody

  • ER-alpha antibody
  • Era antibody
  • ESR antibody
  • ESR1 antibody
  • ESR1_HUMAN antibody
  • ESRA antibody
  • Estradiol receptor antibody
  • Estrogen nuclear receptor alpha antibody
  • Estrogen receptor 1 antibody
  • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
  • Estrogen receptor alpha antibody
  • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
  • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
  • Estrogen receptor alpha delta 4 +49 isoform antibody
  • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
  • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
  • Estrogen receptor alpha E1 E2 1 2 antibody
  • Estrogen receptor alpha E1 N2 E2 1 2 antibody
  • Estrogen receptor antibody
  • ESTRR antibody
  • NR3A1 antibody
  • Nuclear receptor subfamily 3 group A member 1 antibody

  see all

Protocols

• Immunocytochemistry & immunofluorescence protocols