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Our Abpromise guarantee covers the use of ab3576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|ICC||1/100 - 1/1000.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Estrogen Receptor beta peptide (ab5018).|
|Inhibition Assay||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/1000.|
Immunolocalization of ER beta from CHO cells overexpressing the Human protein, using ab3576 (1ug/ml).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"