Anti-Estrogen Receptor beta antibody (ab5784)


  • Product nameAnti-Estrogen Receptor beta antibody
    See all Estrogen Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor beta
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Estrogen Receptor beta aa 467-485.


  • Positive control
    • WB: rat brain lysate IHC: rat cerebellum (paraventricular nucleus and retrochiasmatic nucleus)



Our Abpromise guarantee covers the use of ab5784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
ICC/IF 1/50 - 1/200.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.


  • FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody images

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Estrogen Receptor beta (green) with ab5784 at 1/100. Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum for 15 minutes at room temperature. Cells were incubated with the primary antibody for at least 1 hour at room temperature. A DyLight 488-conjugated goat anti-rabbit IgG (1/200) secondary antibody was used. Nuclei (blue) were stained with Hoechst. 20X magnification.

  • Predicted band size : 59 kDa

    Shows a Western blot of Estrogen Receptor beta on HEK cell extract using ab5784.

References for Anti-Estrogen Receptor beta antibody (ab5784)

ab5784 has not yet been referenced specifically in any publications.

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I'm sending a free of charge vial of ab3576 on the order ***, which we expect to arrive around August 24. Your purchasing agent will receive an email update if there are further delays to expect.


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Thank you again for your call yesterday and for letting us know about the trouble with ab3577.

I've checked with our supply chain and we do expect to have ab3576 back in stock by mid-late August, tentatively August 21. Would this work for you...

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Thank you very much for your call today and for letting us know about the trouble with ab3577.

As we discussed, I will be happy to send a free of charge replacement antibody since this one did not work. I'm checking with our supply chain rega...

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