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Our Abpromise guarantee covers the use of ab5786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Estrogen Receptor beta peptide (ab41764).|
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 2 - 5 µg/ml.|
|IHC-P||Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"