Anti-Estrogen Receptor beta antibody (ab78946)


  • Product nameAnti-Estrogen Receptor beta antibody
    See all Estrogen Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Receptor beta
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of serine 105 (Q-K-SP-P-W).

  • Positive control
    • Extracts from HeLa cells treated with EGF (200ng/ml, 30mins). HeLa cells.



Our Abpromise guarantee covers the use of ab78946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.


  • FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta antibody images

  • All lanes : Anti-Estrogen Receptor beta antibody (ab78946) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with EGF (200ng/ml, 30mins)
    Lane 2 : Extracts from HeLa cells treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • Immunofluorescence analysis of HeLa cells using ab78946 at 1/500 dilution, in the absence or presence of immunising peptide.

References for Anti-Estrogen Receptor beta antibody (ab78946)

ab78946 has not yet been referenced specifically in any publications.

Product Wall

Thank you again for your call yesterday and for letting us know about the trouble with ab3577.

I've checked with our supply chain and we do expect to have ab3576 back in stock by mid-late August, tentatively August 21. Would this work for you...

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Thank you very much for your call today and for letting us know about the trouble with ab3577.

As we discussed, I will be happy to send a free of charge replacement antibody since this one did not work. I'm checking with our supply chain rega...

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Thank you very much for you reply.

I'm sending a free of charge vial of ab3576 on the order ***, which we expect to arrive around August 24. Your purchasing agent will receive an email update if there are further delays to expect.


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