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Our Abpromise guarantee covers the use of ab3577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.5 µg/ml.|
|EMSA||Use at an assay dependent concentration. PubMed: 18790053|
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Estrogen Receptor beta peptide (ab3564).|
|ICC||Use a concentration of 2 - 4 µg/ml.|
|ChIP||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|EMSA||Use a concentration of 1 - 5 µg/ml.|
ab3577 staining Estrogen Receptor beta in human placental sac tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2.5% serum for 30 minutes at 25°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/200 in PBS) at 25°C for 90 minutes. An undiluted HRP-conjugated horse polyclonal was used as the secondary antibody.
ab3577 labelling Estrogen Receptor beta by Immunocytochemistry/Immunofluorescence.
ab3577 at a concentration of 1-2µg/ml staining ER beta in primate amygdala.
Image courtesy of an anonymous Abreview.Red - ab3577 at a 1/100 dilutionGreen - Actin loading controlAll samples loaded as 20µl.
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