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Synthetic peptide corresponding to the N terminal of Human Estrogen Receptor beta was used as an immunogen (UniProt ID: Q92731).
This product is a recombinant rabbit monoclonal antibody.
A trial size is available to purchase for this antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab92306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 59 kDa.|
|IP||1/10 - 1/120.|
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ab92306 (purified) at 1/20 immunoprecipitating Estrogen Receptor beta in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 82-95 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/1000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
ab92306 has not yet been referenced specifically in any publications.