Anti-Estrogen Receptor beta antibody [ERb455] - BSA and Azide free (ab212351)
Key features and details
- Mouse monoclonal [ERb455] to Estrogen Receptor beta - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-Estrogen Receptor beta antibody [ERb455] - BSA and Azide free
See all Estrogen Receptor beta primary antibodies -
Description
Mouse monoclonal [ERb455] to Estrogen Receptor beta - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Estrogen Receptor beta (C terminal). The exact sequence is proprietary.
Database link: Q92731 -
Positive control
- MCF-7 Cells. Human ovarian, breast, bladder, gastric or salivary carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
purified from Bioreactor Concentrates -
Clonality
Monoclonal -
Clone number
ERb455 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
Tissue specificity
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
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Alternative names
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
Images
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human breast carcinoma sections labeling Estrogen Receptor beta with ab212351 at 1 μg/ml.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human ovarian carcinoma sections labelling Estrogen Receptor beta with ab212351 at 1 μg/ml.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human gastric carcinoma sections labelling Estrogen Receptor beta with ab212351 at 1 μg/ml.
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human bladder carcinoma sections labelling Estrogen Receptor beta with ab212351 at 1 μg/ml.
Datasheets and documents
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Datasheet download
References (3)
ab212351 has been referenced in 3 publications.
- Huang S et al. Clinicopathological and Prognostic Significance of Klotho and Estrogen Receptors Expression in Human Hepatocellular Carcinoma. Turk J Gastroenterol 32:828-836 (2021). PubMed: 34787087
- Wang C et al. Interaction of Estradiol and Endoplasmic Reticulum Stress in the Development of Esophageal Carcinoma. Front Endocrinol (Lausanne) 11:410 (2020). PubMed: 32793111
- Deng X et al. Guanxin Danshen Formulation Protects against Myocardial Ischemia Reperfusion Injury-Induced Left Ventricular Remodeling by Upregulating Estrogen Receptor ß. Front Pharmacol 8:777 (2017). PubMed: 29163163