Overview

  • Product nameAnti-Estrogen Receptor beta antibody [PPG5/10]
    See all Estrogen Receptor beta primary antibodies
  • Description
    Mouse monoclonal [PPG5/10] to Estrogen Receptor beta
  • Specificityab27720 recognises estrogen receptor beta 1.
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the C terminus of the human oestrogen receptor beta 1 isoform.

  • Positive control
    • Human ovary

Properties

Applications

Our Abpromise guarantee covers the use of ab27720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
  • Application notesIHC-Fr: Use at an assay dependent dilution.
    IHC-P: neat - 1/20.
    Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 0.1M citrate buffer, pH6.0 is recommended for this purpose.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionNuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
    • Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ER BETA antibody
      • ER-beta antibody
      • Erb antibody
      • ESR B antibody
      • ESR BETA antibody
      • ESR2 antibody
      • ESR2_HUMAN antibody
      • ESRB antibody
      • ESTRB antibody
      • estrogen nuclear receptor beta variant a antibody
      • estrogen nuclear receptor beta variant b antibody
      • estrogen receptor 2 (ER beta) antibody
      • Estrogen receptor 2 antibody
      • estrogen receptor beta 4 antibody
      • Estrogen receptor beta antibody
      • NR3A2 antibody
      • Nuclear receptor subfamily 3 group A member 2 antibody
      see all

    Anti-Estrogen Receptor beta antibody [PPG5/10] images

    • Paraffin embedded human ovary tissue section immunostained for Estrogen receptor beta using monoclonal antibody ab27720

    References for Anti-Estrogen Receptor beta antibody [PPG5/10] (ab27720)

    This product has been referenced in:
    • Torlakovic E  et al. Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score. Hum Pathol 33:646-51 (2002). Read more (PubMed: 12152165) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab27720.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"