Anti-Estrogen Related Receptor alpha antibody (ab93173)

Overview

  • Product name
    Anti-Estrogen Related Receptor alpha antibody
    See all Estrogen Related Receptor alpha primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Related Receptor alpha
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the Center region of Human Estrogen Related Receptor alpha (NP_004442.3).

  • Positive control
    • WB: A375 cell line lysates IHC-P: human kidney carcinoma FC: 293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab93173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated/ sodium citrate pH6 for antigen retrieval.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/100 - 1/500. Predicted molecular weight: 55 kDa.

Target

  • Function
    Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
    Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Err 1 antibody
    • ERR a antibody
    • ERR alpha antibody
    • ERR-alpha antibody
    • Err1 antibody
    • ERR1 protein antibody
    • ERR1_HUMAN antibody
    • ERRa antibody
    • ERRalpha antibody
    • ESRL 1 antibody
    • ESRL1 antibody
    • ESRR A antibody
    • Esrra antibody
    • Estrogen receptor like 1 antibody
    • Estrogen receptor related 1 antibody
    • estrogen receptor related receptor alpha antibody
    • Estrogen receptor-like 1 antibody
    • Estrogen related receptor alpha antibody
    • Estrogen-related receptor alpha antibody
    • Estrra antibody
    • hERR1 antibody
    • NR3B1 antibody
    • Nuclear receptor subfamily 3 group B member 1 antibody
    • Steroid hormone receptor ERR1 antibody
    see all

Images

  • Anti-Estrogen Related Receptor alpha antibody (ab93173) at 1/100 dilution + A375 cell line lysate at 35 µg

    Predicted band size : 55 kDa
  • ab93173, at a 1/50 dilution, staining Estrogen Related Receptor alpha in formalin-fixed and paraffin-embedded Human kidney carcinoma, peroxidase-conjugated to a FITC-conjugated goat-anti-rabbit secondary antibody, followed by DAB staining.
  • ab93173, at a 1/10 dilution, staining Estrogen Related Receptor alpha in 293 cells by flow cytometric analysis (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody used for analysis.

References

ab93173 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93173.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up