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MSSQVVGIEPLYIKAE, corresponding to amino acids 1-16 of Human Estrogen Related Receptor alpha. This sequence is completely conserved between canine, human, mouse and rat. (Peptide available as ab39777.)
Our Abpromise guarantee covers the use of ab16363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 52, 37 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human Estrogen Related Receptor alpha peptide (ab39777).|
|ChIP||Use 0.26 µg for µg of chromatin.|
Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg MCF7 whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16363.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Anti-Rabbit HRP (IgG light chain) (ab99697).
Band: 48kDa: Estrogen Related Receptor alpha.