Anti-Estrogen Related Receptor beta antibody (ab19331)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab19331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18179891
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.

Target

  • Function
    Nuclear receptor, may regulate ESR1 transcriptional activity.
  • Involvement in disease
    Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Err 2 antibody
    • ERR b antibody
    • ERR B2 antibody
    • ERR beta 2 antibody
    • ERR beta antibody
    • ERR beta-2 antibody
    • ERR-beta antibody
    • Err2 antibody
    • ERR2_HUMAN antibody
    • ERRB 2 antibody
    • ERRb antibody
    • ERRB2 antibody
    • ERRbeta 2 antibody
    • ERRbeta antibody
    • ESR L2 antibody
    • ESRL 2 antibody
    • ESRL2 antibody
    • Esrrb antibody
    • Estrogen receptor like 2 antibody
    • Estrogen receptor related 2 antibody
    • Estrogen receptor-like 2 antibody
    • Estrogen-related receptor beta antibody
    • Estrrb antibody
    • Nr3b2 antibody
    • Nuclear receptor ERRB2 antibody
    • Nuclear receptor subfamily 3 group B member 2 antibody
    • Orphan nuclear receptor antibody
    • Steroid hormone receptor ERR 2 antibody
    • Steroid hormone receptor ERR2 antibody
    see all

Images



  • Predicted band size : 48 kDa
    Observed band size : 48 kDa

    Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).

  • ab19331 staining Estrogen Related Receptor Beta protein on mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/200, was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chaudhary S  et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). ChIP . Read more (PubMed: 27089142) »
  • Kunst S  et al. Photoreceptor cells display a daily rhythm in the orphan receptor Esrrß. Mol Vis 21:173-84 (2015). WB . Read more (PubMed: 25737630) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 22 2015

Thank you for your enquiry regarding ab19331.

I can confirm that this antibody has been characterized for IHC-Fr (on frozen tissue), ICC/IF (on cultured cells or cytospin preparations) and in recognizes Estrogen Related Receptor beta in mouse...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Fixative
methanol:acetone 1:1
Permeabilization
No
Blocking step
0.1%BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Nov 24 2011

Thank you for your enquiry regarding our Estrogen Related Receptor beta antibody (ab19331) I can confirm the western blotting procedure used for testing this antibody was as follows: 1) Block membrane with 5 ml of blocking buffer for 1 hour at ...

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I'm very sorry to hear you are experiencing problems with ab19331, ab12986 and ab12987. I have looked at the shipping times of your order and would like to reassure you that there were no delays in the shipping. It is not uncommon that antibodies arri...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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