The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
FunctionOrphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
Tissue specificityExpressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Developmental stageExpressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
Post-translational modificationsSumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity. Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
Nuclear receptor subfamily 3 group B member 3 antibody
Anti-Estrogen Related Receptor gamma antibody images
Western blot - Estrogen Related Receptor gamma antibody (ab49129)
Anti-Estrogen Related Receptor gamma antibody (ab49129) at 0.25 µg/ml + Jurkat cell lysate at 10 µg
Predicted band size : 51 kDa Observed band size : 51 kDa
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Related Receptor gamma antibody (ab49129)
Ab49129, at a concentration of 4-8 µg/ml, positively labelling Estrogen Related Receptor gamma in epithelial cells of bronchiole (indicated by arrows) in paraffin embedded human lung tissue by Immunohistochemistry.
Western blot - Anti-Estrogen Related Receptor gamma antibody (ab49129)
All lanes : Anti-Estrogen Related Receptor gamma antibody (ab49129) at 0.5 µg/ml
Lane 1 : Markers Lane 2 : Zebrafish brain homogenate at 20 µg Lane 3 : Zebrafish heart homogenate at 20 µg Lane 4 : Zebrafish liver homogenate at 20 µg Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg Lane 6 : Mouse Brain Homogenate at 20 µg
Secondary Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution developed using the ECL technique
References for Anti-Estrogen Related Receptor gamma antibody (ab49129)
This product has been referenced in:
Dwivedi SK et al. Bile acid receptor agonist GW4064 regulates PPAR? coactivator-1a expression through estrogen receptor-related receptor a. Mol Endocrinol25:922-32 (2011).
Read more (PubMed: 21493670) »