The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.
Expressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity. Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
Ab49129, at a concentration of 4-8 µg/ml, positively labelling Estrogen Related Receptor gamma in epithelial cells of bronchiole (indicated by arrows) in paraffin embedded human lung tissue by Immunohistochemistry.
Western blot - Anti-Estrogen Related Receptor gamma antibody (ab49129)
All lanes : Anti-Estrogen Related Receptor gamma antibody (ab49129) at 0.5 µg/ml
Lane 1 : Markers Lane 2 : Zebrafish brain homogenate at 20 µg Lane 3 : Zebrafish heart homogenate at 20 µg Lane 4 : Zebrafish liver homogenate at 20 µg Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg Lane 6 : Mouse Brain Homogenate at 20 µg
Secondary Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution Developed using the ECL technique
References for Anti-Estrogen Related Receptor gamma antibody (ab49129)
This product has been referenced in:
Krzysik-Walker SM et al. The Biarylpyrazole Compound AM251 Alters Mitochondrial Physiology Via Proteolytic Degradation of ERRa Mol PharmacolN/A:N/A (2012).
Read more (PubMed: 23066093) »
Dwivedi SK et al. Bile acid receptor agonist GW4064 regulates PPAR? coactivator-1a expression through estrogen receptor-related receptor a. Mol Endocrinol25:922-32 (2011).
Read more (PubMed: 21493670) »