Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366)

Overview

  • Product nameAnti-Estrogen Related Receptor gamma antibody - ChIP Grade
    See all Estrogen Related Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Estrogen Related Receptor gamma - ChIP Grade
  • SpecificityThis antibody detects human Estrogen Related Receptor gamma from transfected cells and shows no cross reactivity to ERR1.
  • Tested applicationsSuitable for: ChIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    DYEAGQHMEDPRR

    , corresponding to amino acids 401-413 of Human Estrogen Related Receptor gamma. This sequence is completely conserved between human, mouse and rat. (Peptide available as ab39779.)

  • Positive control
    • COS7 or HEK293 transfected cells. Human brain, kidney or pancreas.

Properties

Applications

Our Abpromise guarantee covers the use of ab16366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human Estrogen Related Receptor gamma peptide (ab39779). Representing Estrogen Related Receptor gamma from COS7 transfected cells.

Target

  • FunctionOrphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
  • Tissue specificityExpressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stageExpressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
  • Post-translational
    modifications
    Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity.
    Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781L1617 antibody
    • ERR 3 antibody
    • ERR G2 antibody
    • ERR gamma 2 antibody
    • ERR gamma-2 antibody
    • ERR3 antibody
    • ERR3_HUMAN antibody
    • ERRG 2 antibody
    • ERRG2 antibody
    • ERRgamma antibody
    • ESRRG antibody
    • Estrogen receptor related protein 3 antibody
    • Estrogen receptor-related protein 3 antibody
    • Estrogen-related receptor gamma antibody
    • FLJ16023 antibody
    • KIAA0832 antibody
    • NR3B3 antibody
    • Nuclear receptor subfamily 3 group B member 3 antibody
    see all

Anti-Estrogen Related Receptor gamma antibody - ChIP Grade images

  • Lanes 1 - 2 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/500 dilution
    Lane 3 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Mouse kidney cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

References for Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366)

ab16366 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. ERR3 (ab16366) is epitope affinity purified, but it appears there are a few non-specific bands around the 54 kDa range, which is why this product is recommended for cells overexpressing the protein. ERR3 has been used ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"