Anti-Estrogen Sulfotransferase antibody [EST.1] (ab3091)

Overview

  • Product nameAnti-Estrogen Sulfotransferase antibody [EST.1]
    See all Estrogen Sulfotransferase primary antibodies
  • Description
    Mouse monoclonal [EST.1] to Estrogen Sulfotransferase
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Purified rat liver Estrogen Sulfotransferase.

  • Positive control
    • Rat liver tissue sections. Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab3091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
WB
  • Application notesImmunofluorescence / Western Blotting: assay dependent.
    Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4µg/ml for 30 minutes at RT.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    By Western blot, this antibody detects a band of ~ 31 kDa, which corresponds to the predicted molecular weight of Estrogen Sulfotransferase.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
  • Target

    • FunctionMay control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
    • Tissue specificityLiver, intestine and at lower level in the kidney.
    • Sequence similaritiesBelongs to the sulfotransferase 1 family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EST antibody
      • EST-1 antibody
      • EST1 antibody
      • Estrogen sulfotransferase antibody
      • estrogen-preferring antibody
      • estrone sulfotransferase antibody
      • ST1E1 antibody
      • ST1E1_HUMAN antibody
      • STE antibody
      • Sulfotransferase 1E1 antibody
      • Sulfotransferase antibody
      • Sulfotransferase estrogen preferring antibody
      • SULT1E1 antibody
      see all

    References for Anti-Estrogen Sulfotransferase antibody [EST.1] (ab3091)

    ab3091 has not yet been referenced specifically in any publications.

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