Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)

Overview

  • Product nameEstrogen Sulfotransferase overexpression 293T lysate (whole cell)
    See all Estrogen Sulfotransferase lysates
  • General notesab94289 is a 293T cell transfected lysate in which Human Estrogen Sulfotransferase has been transiently over-expressed using a pCMV-Estrogen Sulfotransferase plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: May control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Tissue specificity: Liver, intestine and at lower level in the kidney. Similarity: Belongs to the sulfotransferase 1 family.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Estrogen Sulfotransferase overexpression 293T lysate (whole cell) images

  • ab94289 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Estrogen Sulfotransferase antibody (ab90441) at 1/500 dilution

    Lane 1 : Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Estrogen Sulfotransferase overexpression 293T lysate (whole cell) (ab94289)

ab94289 has not yet been referenced specifically in any publications.

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