Overview

  • Product nameAnti-ESX1 antibody
    See all ESX1 primary antibodies
  • Description
    Rabbit polyclonal to ESX1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: MARGGEDEEN TRSKPEYGTE AENNVGTEGS VPSDDQDREG GGGHEPEQQQ , corresponding to N terminal amino acids 37-86 of Human ESX1

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 45 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

  • FunctionMay coordinately regulate cell cycle progression and transcription during spermatogenesis. Inhibits degradation of polyubiquitinated cyclin A and cyclin B1 and thereby arrests the cell cycle at early M phase. ESXR1-N acts as a transcriptional repressor. Binds to the sequence 5'-TAATGTTATTA-3' which is present within the first intron of the KRAS gene and inhibits its expression. ESXR1-C has the ability to inhibit cyclin turnover.
  • Tissue specificityExpressed in placenta and testis. Expressed in testicular germ cell tumors.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Undergoes proteolytic cleavage; produces a 45 kDa N-terminal homeodomain-containing fragment (ESXR1-N) and a 20 kDa C-terminal fragment (ESXR1-C).
  • Cellular localizationCytoplasm. Nucleus. ESXR1-N localizes specifically to the nucleus while ESXR1-C localizes specifically to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epx antibody
    • ESX1 antibody
    • ESX1_HUMAN antibody
    • ESX1L antibody
    • ESXR1 antibody
    • Etx antibody
    • Extraembryonic antibody
    • Extraembryonic spermatogenesis homeobox 1 antibody
    • homeobox 1 antibody
    • Homeobox protein ESX1-C antibody
    • spermatogenesis antibody
    • Spx1 antibody
    see all

Anti-ESX1 antibody images

  • Anti-ESX1 antibody (ab49109) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References for Anti-ESX1 antibody (ab49109)

ab49109 has not yet been referenced specifically in any publications.

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