Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
  • Tissue specificity
    Broadly expressed, with highest levels in brain.
  • Sequence similarities
    Contains 1 UBA domain.
    Contains 1 UBX domain.
  • Cellular localization
    Cytoplasm. Lipid droplet. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI462440 antibody
    • expressed in T cells and eosinophils in atopic dermatitis antibody
    • faf2 antibody
    • FAF2_HUMAN antibody
    • Fas associated factor family member 2 antibody
    • FAS-associated factor 2 antibody
    • KIAA0887 antibody
    • Protein ETEA antibody
    • Protein expressed in T cells and eosinophils in atopic dermatitis antibody
    • Protein expressed in T-cells and eosinophils in atopic dermatitis antibody
    • UBX domain containing 8 antibody
    • UBX domain protein 3B antibody
    • UBX domain-containing protein 3B antibody
    • UBX domain-containing protein 8 antibody
    • UBXD8 antibody
    • UBXN3B antibody
    see all

Images

  • All lanes : Anti-ETEA antibody (ab14759) at 1 µg/ml

    Lane 1 : Human thymus lysate (in RIPA buffer)
    Lane 2 : Mouse thymus lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

    Detected by chemiluminescence.

  • Anti-ETEA antibody (ab14759) at 1 µg/ml + Cell lysates prepared from Jurkat cells at 35 µg

    Predicted band size : 51 kDa
    Western blot showing ab14759 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.

References

ab14759 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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