Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
Western blot - Anti-ETFA antibody [2B11AE8] (ab110316)
All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml
Lane 1 : Human heart tissue Lane 2 : HepG2 whole cells Lane 3 : Human liver mitochondria Lane 4 : Bovine heart mitochondria Lane 5 : Rat liver mitochondria Lane 6 : Mouse liver mitochondria
IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-ETFA antibody [2B11AE8] (ab110316)
This product has been referenced in:
Davoudi M et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One9:e86767 (2014).
Read more (PubMed: 24466228) »
Pougovkina O et al. Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation. Hum Mol GenetN/A:N/A (2014).
Read more (PubMed: 24516071) »