Overview

  • Product nameAnti-ETFA antibody [2B11AE8]
    See all ETFA primary antibodies
  • Description
    Mouse monoclonal [2B11AE8] to ETFA
  • Tested applicationsIP, WB, ICC/IF, In-Cell ELISA, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human recombinant full length protein ETFA

  • Positive control
    • HeLa cells Human heart tissue HepG2 whole cells Human liver mitochondria Bovine heart mitochondria Rat liver mitochondria Mouse liver mitochondria

Properties

Applications

Our Abpromise guarantee covers the use of ab110316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
ICC/IF Use a concentration of 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Involvement in diseaseDefects in ETFA are the cause of glutaric aciduria type 2A (GA2A) [MIM:231680]; also known as glutaricaciduria IIA. GA2A is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
  • Sequence similaritiesBelongs to the ETF alpha-subunit/FixB family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha ETF antibody
    • Alpha-ETF antibody
    • Electron transfer flavoprotein alpha polypeptide antibody
    • Electron transfer flavoprotein alpha subunit antibody
    • Electron transfer flavoprotein subunit alpha mitochondrial antibody
    • Electron transfer flavoprotein subunit alpha, mitochondrial antibody
    • Electron transferring flavoprotein alpha polypeptide antibody
    • EMA antibody
    • ETFA antibody
    • ETFA_HUMAN antibody
    • GA2 antibody
    • Glutaric aciduria II antibody
    see all

Anti-ETFA antibody [2B11AE8] images

  • Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml

    Lane 1 : Human heart tissue
    Lane 2 : HepG2 whole cells
    Lane 3 : Human liver mitochondria
    Lane 4 : Bovine heart mitochondria
    Lane 5 : Rat liver mitochondria
    Lane 6 : Mouse liver mitochondria

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 35 kDa
  • HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ETFA antibody [2B11AE8] (ab110316)

This product has been referenced in:
  • Davoudi M  et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One 9:e86767 (2014). WB ; Mouse . Read more (PubMed: 24466228) »
  • Pougovkina O  et al. Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 24516071) »

See all 2 Publications for this product

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