The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 35 kDa).
FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Involvement in diseaseGlutaric aciduria 2A
Sequence similaritiesBelongs to the ETF alpha-subunit/FixB family.
DomainDomain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
Post-translational modificationsThe N-terminus is blocked.