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Products:Signal Transduction >> Metabolism >> Mitochondrial
Overview
Product name
Anti-ETFA antibody
See all ETFA products (4) ...
Description
Rabbit polyclonal to ETFA
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig
Immunogen
Synthetic peptide (Human)
Positive control
Fetal heart lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Western blot - Anti-ETFA antibody (ab76426)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Involvement in disease
Defects in ETFA are the cause of glutaric aciduria type 2A (GA2A) [MIM:231680]; also known as glutaricaciduria IIA. GA2A is an autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
Sequence similarities
Belongs to the ETF alpha-subunit/fixB family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP13804
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha ETF antibody
- Alpha-ETF antibody
- Electron transfer flavoprotein alpha polypeptide antibody
see all
Database links
- Entrez Gene: 521892 Cow
- Entrez Gene: 610134 Dog
- Entrez Gene: 2108 Human
- Entrez Gene: 110842 Mouse
- Entrez Gene: 396615 Pig
- Entrez Gene: 300726 Rat
- Omim: 608053 Human
- SwissProt: Q2KJE4 Cow
see all
Anti-ETFA antibody images:
Western blot - Anti-ETFA antibody (ab76426)
Anti-ETFA antibody (ab76426) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal heart lysate at 10 µg
Secondary
HRP-conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
References for Anti-ETFA antibody (ab76426)
ab76426 has not yet been referenced specifically in any publications.
Publishing research using ab76426? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"