The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Tissue specificityAbundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
Involvement in diseaseGlutaric aciduria 2B
Sequence similaritiesBelongs to the ETF beta-subunit/FixA family.
DomainThe recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
Post-translational modificationsMethylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
ab104944 (3.8µg/ml) staining of paraffin embedded Human Liver tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining mitochondrial staining in hepatocytes.
Western blot - ETFB antibody (ab104944)
Anti-ETFB antibody (ab104944) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg Developed using the ECL technique
Predicted band size : 28 kDa Primary incubation was 1 hour.
References for Anti-ETFB antibody (ab104944)
This product has been referenced in:
Zhao Q et al. Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep14:681-8 (2016).
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