Overview

  • Product name
  • Description
    Goat polyclonal to ETFB
  • Specificity
    ab104944 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QVTLEGDKLKVERE

    , corresponding to internal sequence amino acids 152-165 of Human ETFB (NP_001976.1).

  • Positive control
    • Human, Mouse and Rat Liver lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab104944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Tissue specificity
    Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
  • Involvement in disease
    Glutaric aciduria 2B
  • Sequence similarities
    Belongs to the ETF beta-subunit/FixA family.
  • Domain
    The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
  • Post-translational
    modifications
    Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta ETF antibody
    • Beta-ETF antibody
    • Electron transfer flavoprotein beta polypeptide antibody
    • Electron transfer flavoprotein beta subunit antibody
    • Electron transfer flavoprotein subunit beta antibody
    • Electron transferring flavoprotein beta polypeptide antibody
    • etfB antibody
    • ETFB_HUMAN antibody
    • FP585 antibody
    • MADD antibody
    see all

Images

  • ab104944 (3.8µg/ml) staining of paraffin embedded Human Liver tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining mitochondrial staining in hepatocytes.

  • Anti-ETFB antibody (ab104944) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 28 kDa
    Primary incubation was 1 hour.

References

This product has been referenced in:
  • Zhao Q  et al. Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14:681-8 (2016). WB ; Rat . Read more (PubMed: 27222353) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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