Overview

  • Product nameAnti-ETFB antibody
    See all ETFB primary antibodies
  • Description
    Rabbit polyclonal to ETFB
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the C-terminal domain of human ETFB

Applications

Our Abpromise guarantee covers the use of ab73986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 28 kDa.
IHC-P 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.

Target

  • FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Tissue specificityAbundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
  • Involvement in diseaseGlutaric aciduria 2B
  • Sequence similaritiesBelongs to the ETF beta-subunit/FixA family.
  • DomainThe recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
  • Post-translational
    modifications
    Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta ETF antibody
    • Beta-ETF antibody
    • Electron transfer flavoprotein beta polypeptide antibody
    • Electron transfer flavoprotein beta subunit antibody
    • Electron transfer flavoprotein subunit beta antibody
    • Electron transferring flavoprotein beta polypeptide antibody
    • etfB antibody
    • ETFB_HUMAN antibody
    • FP585 antibody
    • MADD antibody
    see all

Anti-ETFB antibody images

  • IHC image of ab73986 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73986, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ETFB antibody (ab73986)

This product has been referenced in:
  • Schmiesing J  et al. Interaction of glutaric aciduria type 1-related glutaryl-CoA dehydrogenase with mitochondrial matrix proteins. PLoS One 9:e87715 (2014). WB . Read more (PubMed: 24498361) »
  • Codarin E  et al. Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues. J Proteome Res 8:2273-84 (2009). Read more (PubMed: 19290626) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"