Overview

  • Product nameAnti-ETFB antibody
    See all ETFB primary antibodies
  • Description
    Rabbit polyclonal to ETFB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-220 of Human ETFB (NP_001976).

  • Positive control
    • MOLT4 whole cell lysate; HeLa cells; 293T, A431, H1299, HepG2 and Raji cell lines

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab97936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 28 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionThe electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • Tissue specificityAbundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
  • Involvement in diseaseGlutaric aciduria 2B
  • Sequence similaritiesBelongs to the ETF beta-subunit/FixA family.
  • DomainThe recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface.
  • Post-translational
    modifications
    Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta ETF antibody
    • Beta-ETF antibody
    • Electron transfer flavoprotein beta polypeptide antibody
    • Electron transfer flavoprotein beta subunit antibody
    • Electron transfer flavoprotein subunit beta antibody
    • Electron transferring flavoprotein beta polypeptide antibody
    • etfB antibody
    • ETFB_HUMAN antibody
    • FP585 antibody
    • MADD antibody
    see all

Anti-ETFB antibody images

  • Anti-ETFB antibody (ab97936) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 28 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97936 at 1/200 dilution. Lower image represents stain merged with DNA probe.

References for Anti-ETFB antibody (ab97936)

This product has been referenced in:
  • Malecki J  et al. Human METTL20 is a mitochondrial lysine methyltransferase that targets the ß subunit of electron transfer flavoprotein (ETFß) and modulates its activity. J Biol Chem 290:423-34 (2015). Read more (PubMed: 25416781) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"