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Abcam's Ethanol Assay Kit provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media. Alcohol oxidase oxidizes ethanol to generate H2O2 which reacts with our probe to generate color (λmax= 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit detects 0.1-10 ppm alcohol (~10-800 nM).
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PLEASE NOTE: Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
Alcohol (ethanol C2H5OH) is among the most widely consumed drinks. Low doses of alcohol may help circulation while heavy alcohol consumption may lead to various forms of disease. Quantitative determination of alcohol finds applications in basic research, drug discovery, clinical studies and fermentation industry processes.
|Ethanol Assay Buffer||WM||1 x 25ml|
|Ethanol Enzyme Mix||Green||1 vial|
|Ethanol Probe (in DMSO, anhydrous)||Red||1 x 200µl|
|Ethanol Standard||Yellow||1 x 500µl|
Our Abpromise guarantee covers the use of ab65343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Ethanol in blood plasma was analyzed using ethanol assay kit (ab65343). Ethanol concentrations were determined 60 minutes after urethane administration in P0/1 mice and rats.
0.5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured fluorimetrically (background subtracted, duplicates; +/- SD).
5 nmol of ethanol was added to diluted human biological fluids (1/10) and spiked samples were measured colourimetrically (background subtracted, duplicates; +/- SD).
Standard curves with background signal subtracted (duplicates; +/- SD).