Overview

  • Product name
  • Description
    Mouse polyclonal to ETHE1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-254 of Human ETHE1

  • Positive control
    • Liver tissue. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab67528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml. Antibody was purified with protein A prior to testing with this application.
    WB: 1/500 - 1/1000. Predicted molecular weight: 28 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Probably plays an important role in metabolic homeostasis in mitochondria. May function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. Suppresses p53-induced apoptosis by preventing nuclear localization of RELA.
    • Tissue specificity
      Ubiquitously expressed.
    • Involvement in disease
      Defects in ETHE1 are a cause of ethylmalonic encephalopathy (EE) [MIM:602473]. EE is an autosomal recessive disorder characterized by neurodevelopmental delay and regression, recurrent petechiae, acrocyanosis, diarrhea, leading to death in the first decade of life. It is also associated with persistent lactic acidemia and ethylmalonic and methylsuccinic aciduria.
    • Sequence similarities
      Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
    • Cellular localization
      Cytoplasm. Nucleus. Mitochondrion matrix. According to PubMed:12398897, it is cytoplasmic and nuclear. According to PubMed:14732903, it is found in the mitochondrial matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ethe1 antibody
      • ETHE1 protein, mitochondrial precursor antibody
      • ETHE1_HUMAN antibody
      • ethylmalonic encephalopathy 1 antibody
      • Ethylmalonic encephalopathy protein 1 antibody
      • hepatoma subtracted clone one antibody
      • Hepatoma subtracted clone one protein antibody
      • HSCO antibody
      • mitochondrial antibody
      • Protein ETHE1 antibody
      • Protein ETHE1 mitochondrial antibody
      • YF13H12 antibody
      see all

    Images

    • Immunofluorescence analysis of HeLa cells using protein A purified ab76528 at 10 ug/ml
    • All lanes : Anti-ETHE1 antibody (ab67528) at 1/500 dilution

      Lane 1 : ETHE1 transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • Anti-ETHE1 antibody (ab67528) at 1/500 dilution + human liver lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

      Predicted band size : 28 kDa
      Observed band size : 26 kDa (why is the actual band size different from the predicted?)

    References

    ab67528 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Cell lysate - whole cell (293T, U2OS, HCT116, Hela)
    Specification
    293T, U2OS, HCT116, Hela
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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