Overview

  • Product name
    Anti-ETS1 antibody [8A8]
    See all ETS1 primary antibodies
  • Description
    Mouse monoclonal [8A8] to ETS1
  • Tested applications
    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Human ETS1 expressed in E. Coli.

  • Positive control
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR118550-11 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab96478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/200 - 1/1000.

Target

  • Function
    Transcription factor.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Post-translational
    modifications
    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • c-ets-1 antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS proto-oncogene 1, transcription factor antibody
    • ETS1 antibody
    • ETS1 oncogene antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • Oncogene ETS1 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

Images

  • All lanes : Anti-ETS1 antibody [8A8] (ab96478) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : ETS1-hIgGFc transfected HEK293 cell lysate


    Predicted band size : 50 kDa
  • ab96478 at 1/200 dilution staining ETS1 in Jurkat cells by Flow Cytometry (shown in green), negative control in purple.
  • ab96478 at 1/200 dilution staining ETS1 in NIH3T3 cells by Immunofluorescence (green). DRAQ5 fluorescent DNA dye (blue).

References

ab96478 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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