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Rabbit polyclonal to ETS1
Predicted to work with:
Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Medaka fish
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human ETS1 (NP_005229.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab124282 was affinity purified using an epitope specific to ETS1 immobilized on solid support .
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
Use at 2-10 µg/mg of lysate.
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Information by UniProt
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
C ets 1 protein antibody
Western blot - Anti-ETS1 antibody (ab124282)
All lanes : Anti-ETS1 antibody (ab124282) at 0.1 µg/ml Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Developed using the ECL technique. Exposure time: 3 minutes
Immunoprecipitation - Anti-ETS1 antibody (ab124282)
ab124282 at 1 µg/ml staining ETS1 in Jurkat whole cell lysate immunoprecipitated using ab124282 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 50 kDa.
Exposure time: 30 seconds
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"