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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab124282 was affinity purified using an epitope specific to ETS1 immobilized on solid support .
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000.
Use at 2-10 µg/mg of lysate.
Function Transcription factor.
Sequence similarities Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Post-translational modifications Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity. Ubiquitinated; which induces proteasomal degradation.
Cellular localization Nucleus.
Information by UniProt
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
C ets 1 protein antibody
Anti-ETS1 antibody images
Western blot - Anti-ETS1 antibody (ab124282)
All lanes : Anti-ETS1 antibody (ab124282) at 0.1 µg/ml Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Developed using the ECL technique Exposure time : 3 minutes
Immunoprecipitation - Anti-ETS1 antibody (ab124282)
ab124282 at 1 µg/ml staining ETS1 in Jurkat whole cell lysate immunoprecipitated using ab124282 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 50 kDa.
Exposure time: 30 seconds
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