Anti-ETS1 antibody [mAbcam63302] (ab63302)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab63302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionTranscription factor.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • Post-translational
    modifications
    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS1 antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

Anti-ETS1 antibody [mAbcam63302] images

  • All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible isoform).

    Exposure time : 2 minutes

References for Anti-ETS1 antibody [mAbcam63302] (ab63302)

ab63302 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 32 µg
Gel Running Conditions Reduced Denaturing (10% gel, semi-dry transfer)
Sample Chicken Cell lysate - whole cell (LMH cells overexpressing chicken Ets1A and B)
Specification LMH cells overexpressing chicken Ets1A and B
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"