Anti-ETS1 associated protein II antibody (ab99279)

Overview

  • Product name
    Anti-ETS1 associated protein II antibody
    See all ETS1 associated protein II primary antibodies
  • Description
    Rabbit polyclonal to ETS1 associated protein II
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 50-100 of Human ETS1 associated protein II (NP_057698.2).

  • Positive control
    • HeLa and 293T whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the CCR4/nocturin family.
  • Cellular localization
    Nucleus. Nucleus > PML body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5'-Tyr-DNA phosphodiesterase antibody
    • 5'-tyrosyl-DNA phosphodiesterase antibody
    • AD 022 antibody
    • AD022 antibody
    • dJ30M3.3 antibody
    • EAP 2 antibody
    • EAP II antibody
    • EAP2 antibody
    • EAPII antibody
    • ETS 1 associated protein 2 antibody
    • ETS 1 associated protein II antibody
    • ETS1 associated protein 2 antibody
    • ETS1-associated protein 2 antibody
    • ETS1-associated protein II antibody
    • MGC111021 antibody
    • MGC9099 antibody
    • RP1-30M3.3 antibody
    • tdp2 antibody
    • TRAF and TNF receptor associated protein antibody
    • TRAF and TNF receptor-associated protein antibody
    • TTRAP antibody
    • TYDP2_HUMAN antibody
    • Tyr DNA phosphodiesterase 2 antibody
    • Tyr-DNA phosphodiesterase 2 antibody
    • Tyrosyl DNA phosphodiesterase 2 antibody
    • Tyrosyl-DNA phosphodiesterase 2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling ETS1 associated protein II with ab99279 at 1/200 (1µg/mg). Detection: DAB.
  • All lanes : Anti-ETS1 associated protein II antibody (ab99279) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 41 kDa
  • ab99279, at 0.4 µg/ml, staining ETS1 associated protein II in HeLa whole cell lysate immunoprecipitated using ab99279 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab99279 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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