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Rabbit polyclonal to ETS2
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human ETS2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P15036
This antibody gave a positive signal in Human Heart and Kidney tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Information by UniProt
C ets 2 protein antibody
Western blot - Anti-ETS2 antibody (ab153652)
All lanes :
Anti-ETS2 antibody (ab153652) at 1 µg/ml
Lane 1 :
Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 :
Kidney (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
15 kDa (possible non-specific binding), 46 kDa (possible non-specific binding)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab153652 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"