Overview

  • Product name
  • Description
    Rabbit polyclonal to ETS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ETS2 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P15036

  • Positive control
    • This antibody gave a positive signal in Human Heart and Kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab153652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

Images

  • All lanes : Anti-ETS2 antibody (ab153652) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 15 kDa (possible non-specific binding), 46 kDa (possible non-specific binding)


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab153652 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Shin J  et al. Depletion of ERK2 but not ERK1 abrogates oncogenic Ras-induced senescence. Cell Signal 25:2540-7 (2013). WB ; Mouse . Read more (PubMed: 23993963) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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