Anti-ETS2 (phospho T72) antibody (ab153653)

Overview

  • Product name
    Anti-ETS2 (phospho T72) antibody
    See all ETS2 primary antibodies
  • Description
    Rabbit polyclonal to ETS2 (phospho T72)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Horse, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ETS2 aa 50-150 (phospho T72) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P15036

  • Positive control
    • This antibody gave a positive signal in Human Kidney tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab153653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 53 kDa).
ELISA Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-ETS2 (phospho T72) antibody (ab153653) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203) with Immunising peptide at 1 µg/ml
    Lane 3 : Human kidney tissue lysate - total protein (ab30203) with Control peptide at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)


    Exposure time: 8 minutes


    The predicted molecular weight of ETS2 is 53 kDa (SwissProt), however we expect to observe a banding pattern at 70 kDa.

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab153653 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • ab153653 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

References

ab153653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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