Recombinant Anti-ETV2 antibody [EPR5229(3)] (ab181847)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5229(3)] to ETV2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ETV2 antibody [EPR5229(3)]
See all ETV2 primary antibodies -
Description
Rabbit monoclonal [EPR5229(3)] to ETV2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC cell lysate of lent-virally expressed Human ETV2 and Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2. HUVEC cells expressing exogenous Human ETV2.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5229(3) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
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ICC/IF |
1/500.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa). |
ICC/IF
1/500. |
Target
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Relevance
ETV2 is a member of the ETS family of transcription factors. It has been proposed that ETV2 acts downstream of BMP, notch and wnt signalling in blood and vessel specification. ER71 has also shown to be important for Flk1 expression as well as hematopoietic and vascular development. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2116 Human
- Entrez Gene: 14008 Mouse
- Omim: 609358 Human
- SwissProt: O00321 Human
- SwissProt: P41163 Mouse
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Alternative names
- ER71 antibody
- Ets related protein 71 antibody
- ETS translocation variant 2 antibody
see all
Images
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All lanes : Anti-ETV2 antibody [EPR5229(3)] (ab181847) at 1/10000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate of lent-virally expressed Human ETV2
Lane 3 : Human bone marrow stromal cell lysate
Lane 4 : Human bone marrow stromal cell lysate lent-virally expressed mouse ETV2
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells with exogenous Human ETV2 (upper panels) or without (lower panels), labeling ETV2 with ab181847 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®488) was used at a 1/200. In blue DAPI staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab181847 has been referenced in 5 publications.
- Sasaki M et al. Introduction of Mutant GNAQ into Endothelial Cells Induces a Vascular Malformation Phenotype with Therapeutic Response to Imatinib. Cancers (Basel) 14:N/A (2022). PubMed: 35053574
- Tan J et al. Molecular Subtypes Based on the Stemness Index Predict Prognosis in Glioma Patients. Front Genet 12:616507 (2021). PubMed: 33732284
- Li J et al. Upregulation of ETV2 Expression Promotes Endothelial Differentiation of Human Dental Pulp Stem Cells. Cell Transplant 30:963689720978739 (2021). PubMed: 33522307
- Palikuqi B et al. Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis. Nature 585:426-432 (2020). PubMed: 32908310
- Kabir AU et al. Requisite endothelial reactivation and effective siRNA nanoparticle targeting of Etv2/Er71 in tumor angiogenesis. JCI Insight 3:N/A (2018). PubMed: 29669933