Overview

  • Product nameAnti-ETV3 antibody [ETV3F4D10]
    See all ETV3 primary antibodies
  • Description
    Mouse monoclonal [ETV3F4D10] to ETV3
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa whole cell lysate, HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab50751 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (95% approximately) fetal bovine serum. Filtered through a 0.22µm membrane
  • ClonalityMonoclonal
  • Clone numberETV3F4D10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10.
WB 1/100. Predicted molecular weight: 57 kDa.

Target

  • FunctionTranscriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI414410 antibody
    • bA110J1.4 antibody
    • ETS domain transcriptional repressor PE1 antibody
    • ETS family transcriptional repressor antibody
    • ETS translocation variant 3 antibody
    • ets variant 3 antibody
    • ets variant gene 3 antibody
    • ets variant gene 3, ETS family transcriptional repressor antibody
    • ETV3 antibody
    • ETV3_HUMAN antibody
    • ME PE1 antibody
    • METS antibody
    • Mitogenic Ets transcriptional suppressor antibody
    • Oncogene PE1 antibody
    • PE 1 antibody
    • PE-1 antibody
    • PE1 antibody
    see all

Anti-ETV3 antibody [ETV3F4D10] images

  • Anti-ETV3 antibody [ETV3F4D10] (ab50751) at 1/100 dilution + HeLa whole cell lysate

    Secondary
    Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Ab50751 at 1/10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution.

References for Anti-ETV3 antibody [ETV3F4D10] (ab50751)

ab50751 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50751.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"