Overview

  • Product name
    Anti-ETV3 antibody [ETV3F4D10]
    See all ETV3 primary antibodies
  • Description
    Mouse monoclonal [ETV3F4D10] to ETV3
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control
    • HeLa whole cell lysate, HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab50751 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (95% approximately) fetal bovine serum. Filtered through a 0.22µm membrane
  • Clonality
    Monoclonal
  • Clone number
    ETV3F4D10
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10.
WB 1/100. Predicted molecular weight: 57 kDa.

Target

  • Function
    Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI414410 antibody
    • bA110J1.4 antibody
    • ETS domain transcriptional repressor PE1 antibody
    • ETS family transcriptional repressor antibody
    • ETS translocation variant 3 antibody
    • ets variant 3 antibody
    • ets variant gene 3 antibody
    • ets variant gene 3, ETS family transcriptional repressor antibody
    • ETV3 antibody
    • ETV3_HUMAN antibody
    • ME PE1 antibody
    • METS antibody
    • Mitogenic Ets transcriptional suppressor antibody
    • Oncogene PE1 antibody
    • PE 1 antibody
    • PE-1 antibody
    • PE1 antibody
    see all

Anti-ETV3 antibody [ETV3F4D10] images

  • Anti-ETV3 antibody [ETV3F4D10] (ab50751) at 1/100 dilution + HeLa whole cell lysate

    Secondary
    Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 57 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Ab50751 at 1/10 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution.

References for Anti-ETV3 antibody [ETV3F4D10] (ab50751)

ab50751 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab50751.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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