Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab54705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTranscriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'.
    • Tissue specificityUbiquitous.
    • Involvement in diseaseNote=A chromosomal aberration involving ETV6 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with PDGFRB. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
      Note=Chromosomal aberrations involving ETV6 are found in a form of acute myeloid leukemia (AML). Translocation t(12;22)(p13;q11) with MN1; translocation t(4;12)(q12;p13) with CHIC2.
      Note=Chromosomal aberrations involving ETV6 are found in childhood acute lymphoblastic leukemia (ALL). Translocations t(12;21)(p12;q22) and t(12;21)(p13;q22) with RUNX1/AML1.
      Note=A chromosomal aberration involving ETV6 is found in a form of pre-B acute myeloid leukemia. Translocation t(9;12)(p24;p13) with JAK2.
      Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS) with basophilia. Translocation t(5;12)(q31;p13) with ACSL6.
      Note=A chromosomal aberration involving ETV6 is found in acute eosinophilic leukemia (AEL). Translocation t(5;12)(q31;p13) with ACSL6.
      Note=A chromosomal aberration involving ETV6 is found in myelodysplastic syndrome (MDS). Translocation t(1;12)(p36.1;p13) with MDS2.
      Defects in ETV6 are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving ETV6 is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with PDGFRB on chromosome 5 creating an ETV6-PDGFRB fusion protein.
      Defects in ETV6 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
      Note=A chromosomal aberration involving ETV6 is found in acute lymphoblastic leukemia. Translocation t(9;12)(p13;p13) with PAX5.
    • Sequence similaritiesBelongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ETS related protein Tel1 antibody
      • ETS translocation variant 6 antibody
      • Ets variant 6 antibody
      • Ets variant gene 6 (TEL oncogene) antibody
      • ETS-related protein Tel1 antibody
      • ETV 6 antibody
      • ETV6 antibody
      • ETV6_HUMAN antibody
      • Tel antibody
      • TEL oncogene antibody
      • TEL/ABL antibody
      • TEL1 antibody
      • TEL1 oncogene antibody
      • Transcription factor ETV 6 antibody
      • Transcription factor ETV6 antibody
      see all

    Anti-ETV6 / Tel antibody images

    • ETV6 / Tel antibody (ab54705) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.


    • Predicted band size : 53 kDa
      ETV6 / Tel antibody (ab54705) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

    References for Anti-ETV6 / Tel antibody (ab54705)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    It isnt necessary to use a secondary antibody for ChIP. A primary is used for isolating chromatin and then PCR based methods or direct sequencing is used for detection. I have attached a protocol summary.

    http://www.abcam.com/index.html?page...

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    Thank you for your enquiry and your interest in our products.

    I have conducted a search for you at our website and found 5 antibodies against ETV6 / Tel:

    ab23465,

    ab54705,

    ab64909,

    ab74361,

    ab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"