• Product nameAnti-EVI1 antibody
    See all EVI1 primary antibodies
  • Description
    Rabbit polyclonal to EVI1
  • SpecificityAb28457 recognises Ecotropic virus integration 1 site protein(EVI1)
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide derived from residues 964 - 1013 of human EVI1.


  • Positive control
    • WB: fetal lung lysate. IHC-P: human intestine tissue.



Our Abpromise guarantee covers the use of ab28457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 118 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.
ICC/IF Use at an assay dependent concentration. PubMed: 21493867


  • RelevanceEVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • AML1 EVI1 antibody
    • Ecotropic viral integration site 1 antibody
    • Ecotropic virus integration 1 site protein antibody
    • Ecotropic virus integration site 1 protein homolog antibody
    • EVI 1 antibody
    • EVI1 antibody
    • MDS1 and EVI1 complex locus antibody
    • MDS1 and EVI1 complex locus protein EVI1 antibody
    • MDS1 and EVI1 complex locus protein MDS1 antibody
    • MDS1 antibody
    • MDS1 EVI1 antibody
    • MGC163392 antibody
    • MGC97004 antibody
    • Myelodysplasia syndrome 1 antibody
    • Myelodysplasia syndrome 1 protein antibody
    • Myelodysplasia syndrome-associated protein 1 antibody
    • Oncogene EVI1 antibody
    • PRDM3 antibody
    • Zinc finger protein Evi1 antibody
    see all

Anti-EVI1 antibody images

  • Anti-EVI1 antibody (ab28457) at 0.5 µg/ml + fetal lung lysate at 10 µg

    HRP conjugated anti rabbit IgG at 1/50000 dilution

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling EVI1 with ab28457 at 4-8µg/ml. Cells with positive label: epithelial cells of intestinal villus (indicated with arrows). Magnification: 400X.

  • ICC/IF image of ab28457 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-EVI1 antibody (ab28457)

This product has been referenced in:
  • Koos B  et al. The transcription factor evi-1 is overexpressed, promotes proliferation, and is prognostically unfavorable in infratentorial ependymomas. Clin Cancer Res 17:3631-7 (2011). WB, ICC/IF, IHC-P ; Human . Read more (PubMed: 21493867) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Axolotl (Ambystoma mexicanum) Tissue sections (tail)
Permeabilization Yes - 1% Triton X-100
Specification tail
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde

Gufa Lin

Verified customer

Submitted Dec 29 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain tumor (Ependymoma))
Specification Brain tumor (Ependymoma)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH=9
Permeabilization No
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 0.5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 (Mouse embryonic fibroblast cell line))
Loading amount 100 µg
Specification NIH 3T3 (Mouse embryonic fibroblast cell line)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Dec 23 2010