Overview

  • Product nameAnti-EVI1 antibody
    See all EVI1 primary antibodies
  • Description
    Rabbit polyclonal to EVI1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 360-409 (HPSVGDNKPV ELQPERSSEE RPFEKISDQS ESSDLDDVST PSGSDLETTS) of Human EVI1, NP_005232.

  • Positive control
    • Human fetal brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 118 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceEVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • AML1 EVI1 antibody
    • Ecotropic viral integration site 1 antibody
    • Ecotropic virus integration 1 site protein antibody
    • Ecotropic virus integration site 1 protein homolog antibody
    • EVI 1 antibody
    • EVI1 antibody
    • MDS1 and EVI1 complex locus antibody
    • MDS1 and EVI1 complex locus protein EVI1 antibody
    • MDS1 and EVI1 complex locus protein MDS1 antibody
    • MDS1 antibody
    • MDS1 EVI1 antibody
    • MGC163392 antibody
    • MGC97004 antibody
    • Myelodysplasia syndrome 1 antibody
    • Myelodysplasia syndrome 1 protein antibody
    • Myelodysplasia syndrome-associated protein 1 antibody
    • Oncogene EVI1 antibody
    • PRDM3 antibody
    • Zinc finger protein Evi1 antibody
    see all

Anti-EVI1 antibody images

  • Anti-EVI1 antibody (ab86306) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 118 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-EVI1 antibody (ab86306)

ab86306 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (GM-CSF derived macrophages 8 days old)
Loading amount 15 µg
Specification GM-CSF derived macrophages 8 days old
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"