Recombinant Anti-EVI1 antibody [EPR5996] (ab124934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5996] to EVI1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-EVI1 antibody [EPR5996]
See all EVI1 primary antibodies -
Description
Rabbit monoclonal [EPR5996] to EVI1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human EVI1 aa 300-400. The exact sequence is proprietary.
-
Positive control
- HeLa and SKOV 3 cell lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5996 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa).
|
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa). |
Target
-
Relevance
EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 2122 Human
- Omim: 165215 Human
- SwissProt: Q03112 Human
- SwissProt: Q13465 Human
-
Alternative names
- AML1 EVI 1 antibody
- AML1 EVI 1 fusion protein antibody
- AML1 EVI1 antibody
see all
Images
-
All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKOV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 145,200 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible cleavage fragment)
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab124934 has been referenced in 3 publications.
- Luciani GM et al. Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression. Exp Hematol 69:27-36 (2019). PubMed: 30352278
- Ma H et al. PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma. Cell Death Dis 10:166 (2019). PubMed: 30778048
- Ivanochko D et al. Direct interaction between the PRDM3 and PRDM16 tumor suppressors and the NuRD chromatin remodeling complex. Nucleic Acids Res N/A:N/A (2018). PubMed: 30462309