Recombinant
RabMAb

Anti-EVI1 antibody [EPR5996] (ab124934)

Overview

  • Product name
    Anti-EVI1 antibody [EPR5996]
    See all EVI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5996] to EVI1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in EVI1 (Human)

  • Positive control
    • HeLa and SKOV 3 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance
      EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • AML1 EVI 1 antibody
      • AML1 EVI 1 fusion protein antibody
      • AML1 EVI1 antibody
      • Ecotropic viral integration site 1 antibody
      • Ecotropic virus integration 1 site protein antibody
      • Ecotropic virus integration site 1 protein homolog antibody
      • EVI 1 antibody
      • EVI1 antibody
      • MDS1 and EVI1 complex locus antibody
      • MDS1 and EVI1 complex locus protein EVI1 antibody
      • MDS1 and EVI1 complex locus protein MDS1 antibody
      • MDS1 antibody
      • MDS1 EVI1 antibody
      • MGC163392 antibody
      • MGC97004 antibody
      • Myelodysplasia syndrome 1 antibody
      • Myelodysplasia syndrome 1 protein antibody
      • Myelodysplasia syndrome-associated protein 1 antibody
      • Oncogene EVI1 antibody
      • PRDM3 antibody
      • Zinc finger protein Evi1 antibody
      see all

    Images

    • All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SKOV 3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit at 1/2000 dilution

      Predicted band size : 118 kDa
      Observed band size : 145,200 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 105 kDa (possible cleavage fragment).

    References

    ab124934 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124934.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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