Overview

  • Product name
  • Description
    Rabbit polyclonal to EVI5
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    A region between residue 525 and 575 of human EVI5. (NP_005656.4)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.


    Is unsuitable for WB.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      EVI5 is a novel centrosomal protein with a complex expression pattern and subcellular localization, possibly involved in centrosome stability and dynamics. It may act as an oncogene.
    • Cellular localization
      Nucleus, centrosome.
    • Database links
    • Alternative names
      • ecotropic viral integration site 5 antibody
      • NB4S antibody
      • Neuroblastoma stage 4S gene antibody

    Images

    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70790 at 3 µg/mg lysate for IP.
      Detection: Chemiluminescence with an exposure time of 1 second.

    References

    ab70790 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Axolotl (Ambystoma mexicanum) Tissue sections (limb)
    Permeabilization
    Yes - 0.1% tween-20
    Specification
    limb
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Gufa Lin

    Verified customer

    Submitted Jun 29 2015

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Xenopus laevis Tissue sections (limb)
    Permeabilization
    Yes - 0.1% tween-20
    Specification
    limb
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Gufa Lin

    Verified customer

    Submitted Jun 08 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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