Overview

  • Product name
  • Description
    Mouse monoclonal to EWSR1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment: SDNSAIYVQG LNDSVTLDDL ADFFKQCGVV KMNKRTGQPM IHIYLDKETG KPKGDATVSY EDPPTAKAAV EWFDGKDFQG SKLKVSLARK KPPMNS, corresponding to amino acids 358-454 of Human EWSR1

  • Positive control
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab54708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Target

  • Function
    Might normally function as a repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in EWSR1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=Chromosomal aberrations involving EWSR1 are found in patients with Ewing sarcoma. Translocation t(11;22)(q24;q12) with FLI1; translocation t(7;22)(p22;q12) with ETV1; translocation t(21;22)(q22;q12) with ERG; translocation t(9;22)(q22-31;q11-12) with NR4A3. Translocation t(2;21;22)(q23;q22;q12) that forms a EWSR1-FEV fusion protein with potential oncogenic activity.
    Note=A chromosomal aberration involving EWSR1 is associated with desmoplastic small round cell tumor (DSRCT). Translocation t(11;22)(p13;q12) with WT1.
    Note=A chromosomal aberration involving EWSR1 is associated with malignant melanoma of soft parts (MMSP). Translocation t(12;22)(q13;q12) with ATF-1. Malignant melanoma of soft parts, also known as soft tissue clear cell sarcoma, is a rare tumor developing in tendons and aponeuroses.
    Note=A chromosomal aberration involving EWSR1 is associated with small round cell sarcoma. Translocation t(11;22)(p36.1;q12) with PATZ1.
    Defects in EWSR1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=Chromosomal aberrations involving EWSR1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;22)(q13;q12) with ATF1 generates a chimeric EWSR1/ATF1 protein. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
    Note=EFPS arise due to chromosomal translocations in which EWSR1 is fused to a variety of cellular transcription factors. EFPS are very potent transcriptional activators dependent on the EAD and a C-terminal DNA-binding domain contributed by the fusion partner. The spectrum of malignancies associated with EFPS are thought to arise via EFP-induced transcriptional deregulation, with the tumor phenotype specified by the EWSR1 fusion partner and cell type. Transcriptional repression of the transforming growth factor beta type II receptor (TGF beta RII) is an important target of the EWS-FLI1, EWS-ERG, or EWS-ETV1 oncogene.
  • Sequence similarities
    Belongs to the RRM TET family.
    Contains 1 IQ domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    EWS activation domain (EAD) functions as a potent activation domain in EFPS. EWSR1 binds POLR2C but not POLR2E or POLR2G, whereas the isolated EAD binds POLR2E and POLR2G but not POLR2C. Cis-linked RNA-binding domain (RBD) can strongly and specifically repress trans-activation by the EAD.
  • Post-translational
    modifications
    Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266.
    Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.
  • Cellular localization
    Nucleus. Cytoplasm. Cell membrane. Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • bK984G1.4 antibody
    • bK984G1.4 Ewing sarcoma breakpoint region 1 protein antibody
    • Ewing sarcoma breakpoint region 1 antibody
    • Ewing sarcoma breakpoint region 1 protein antibody
    • Ewings sarcoma EWS Fli1 type 1 oncogene antibody
    • EWS antibody
    • EWS oncogene antibody
    • EWS RNA binding protein 1 antibody
    • EWS_HUMAN antibody
    • EWSR 1 antibody
    • Ewsr1 antibody
    • EWSR1 protein antibody
    • RNA binding protein EWS antibody
    • RNA-binding protein EWS antibody
    see all

Images

  • ICC/IF image of ab54708 stained hepG2 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • EWSR1 antibody (ab54708) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human urinary bladder.
  • Western blot against tagged recombinant protein immunogen using ab54708 EWSR1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

References

This product has been referenced in:
  • Parvanov ED  et al. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. Mol Biol Cell 28:488-499 (2017). Read more (PubMed: 27932493) »
  • Duggimpudi S  et al. The cell cycle regulator CCDC6 is a key target of RNA-binding protein EWS. PLoS One 10:e0119066 (2015). Read more (PubMed: 25751255) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK293 expressing HA-tagged EWSR1)
Total protein in input
2e+006 cells
Specification
HEK293 expressing HA-tagged EWSR1
Immuno-precipitation step
Other - Dynabeads
Username

Abcam user community

Verified customer

Submitted Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
30 µg
Specification
HEK293
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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