Overview

  • Product name
  • Description
    Rabbit polyclonal to EXD2
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to amino acids 335-442 of Human EXD2

  • Positive control
    • RT-4, U-251 MG, Human stomach tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 56 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. pH 6.0

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121236 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-EXD2 antibody (ab121236) at 1/250 dilution

    Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human Plasma lysate
    Lane 5 : Human Liver Lysate
    Lane 6 : Human tonsil lysate


    Predicted band size : 56 kDa
  • Immunohistochemical staining of paraffin embedded Human stomach, using ab121236, shows strong cytoplasmic and membranous positivity in glandular cells.

References

ab121236 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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