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Rabbit polyclonal to EXD2
Predicted to work with:
Recombinant fragment, corresponding to amino acids 335-442 of Human EXD2
RT-4, U-251 MG, Human stomach tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/250 - 1/500. Predicted molecular weight: 56 kDa.
1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. pH 6.0
Belongs to the EXD2 family.
Contains 1 3'-5' exonuclease domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-EXD2 antibody (ab121236)
Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab121236 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-EXD2 antibody (ab121236)
All lanes : Anti-EXD2 antibody (ab121236) at 1/250 dilution Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2 : RT-4 cell lysate Lane 3 : U-251 MG cell lysate Lane 4 : Human Plasma lysate Lane 5 : Human Liver Lysate Lane 6 : Human tonsil lysate Predicted band size : 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXD2 antibody (ab121236)
Immunohistochemical staining of paraffin embedded Human stomach, using ab121236, shows strong cytoplasmic and membranous positivity in glandular cells.
has not yet been referenced specifically in any publications.
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