Overview

  • Product name
    Anti-Exendin 4 antibody [20]
    See all Exendin 4 primary antibodies
  • Description
    Mouse monoclonal [20] to Exendin 4
  • Specificity
    This antibody recognises exendin 4. No cross reactivity with human GLP1, GLP2 or glucagon.
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with Heloderma suspectum (Gila monster). Not yet tested in other species.
  • Immunogen

    Carrier coupled exendin 4.

  • Epitope
    The epitope is located in the region of amino acids 9 to 39 of exendin 4.
  • General notes
    The fusion partner was SP2mIL6.

Properties

Applications

Our Abpromise guarantee covers the use of ab23407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000. The antibody binds to exendin 4 when coated on ELISA wells and reacts specifically with exendin 4 in solution giving a Ka of 1.6 x 10E8 in an inhibition ELISA. The binding between ab23407 and exendin 4 is disrupted by 4.5 M MgCl2.

Target

  • Relevance
    Exendin 4 is a 39 amino acid peptide found in venom from the Gila monster Helicoderma suspectum. It is a member of the glucagon secretin family of peptide hormones and neuropeptides. Exendin 4 is a potent agonist of the GLP1 receptor and hence a potent stimulator of insulin secretion.
  • Cellular localization
    Secreted
  • Alternative names
    • Exenatide antibody
    • Exendin4 antibody

Anti-Exendin 4 antibody [20] images

  • The concentration of exendin-4 secreted by recombinant L. paracasei was measured by indirect ELISA. Standard exendin-4 (0.2 μg/ml) was used as the coated antigen. Equal volumes (100 μl) of ab23407 (1/4000) and standard exendin-4 (0–100 nmol/l) or culture supernatant samples of L. paracasei L14/pMG76e-exendin-4, preincubated overnight at 4°C, were applied to detect the captured exendin-4. Horseradish peroxidase-conjugated rabbit anti-mouse IgG (100 μl) was used at a 1:10,000 dilution and revealed by reaction with TMBS substrate. Absorption was determined at 450 nm and 630 nm by Multiskan MK3 ELISA plate reader. The exendin-4 concentration was calculated by the standard curve method.

References for Anti-Exendin 4 antibody [20] (ab23407)

This product has been referenced in:
  • Zeng Z  et al. Heterologous Expression and Delivery of Biologically Active Exendin-4 by Lactobacillus paracasei L14. PLoS One 11:e0165130 (2016). Read more (PubMed: 27764251) »

See 1 Publication for this product

Product Wall

Application
ELISA
Specification
Synthesized peptide
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3%
Type
Sandwich
Username

Mr. Gerald Nakamura

Verified customer

Submitted Apr 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up