Anti-EXOC5 antibody - C-terminal (ab176009)

Overview

  • Product name
    Anti-EXOC5 antibody - C-terminal
  • Description
    Rabbit polyclonal to EXOC5 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EXOC5 aa 46-75 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O00471

  • Positive control
    • NCI-H460 lysate, Human lung carcinoma tissue and NCI-H460 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176009 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 82 kDa.

Target

  • Relevance
    EXOC5 is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • AI447711 antibody
    • AI448003 antibody
    • DKFZp666H126 antibody
    • EXOC5 antibody
    • exocyst complex component 5 antibody
    • Exocyst complex component Sec10 antibody
    • Gm76 antibody
    • HSEC10 antibody
    • p71 component of rsec6/8 secretory complex antibody
    • PRO1912 antibody
    • SEC10 antibody
    • SEC10 like 1 antibody
    • SEC10L1 antibody
    • SEC10P antibody
    see all

Images

  • Anti-EXOC5 antibody - C-terminal (ab176009) at 1/1000 dilution + NCI-H460 lysate at 35 µg

    Predicted band size: 82 kDa

  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling EXOC5 with ab176009 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using ab176009 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab176009 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab176009.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up