Synthetic peptide within Human EXOC5 aa 46-75 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: O00471
Our Abpromise guarantee covers the use of ab176009 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 82 kDa.|
Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling EXOC5 with ab176009 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram) using ab176009 at a 1/10 dilution. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab176009 has not yet been referenced specifically in any publications.