The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionComponent of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
Sequence similaritiesBelongs to the EXO70 family.
DomainThe N-terminus is involved in SEC8 and ARHQ binding. The C-terminus is required for translocation to the plasma membrane.
Cellular localizationCytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
ab115545, at 5µg/ml, staining EXOC7 in Formalin-fixed, Paraffin-embedded Human Adrenal tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-EXOC7 antibody (ab115545)
Anti-EXOC7 antibody (ab115545) at 0.5 µg/ml + Human Lung lysate at 35 µg Developed using the ECL technique
Predicted band size : 83 kDa
References for Anti-EXOC7 antibody (ab115545)
has not yet been referenced specifically in any publications.
Publishing research using ab115545? Please let us know so that we can cite the reference in this datasheet.
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