Overview

  • Product name
  • Description
    Rabbit polyclonal to EXOC7
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human EXOC7 (NP_001138769.1).

  • Positive control
    • HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 83 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
  • Sequence similarities
    Belongs to the EXO70 family.
  • Domain
    The N-terminus is involved in SEC8 and ARHQ binding.
    The C-terminus is required for translocation to the plasma membrane.
  • Cellular localization
    Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 5 3p antibody
    • DKFZp686J04253 antibody
    • EX070 antibody
    • EXO70 antibody
    • Exo70p antibody
    • EXOC 1 antibody
    • EXOC 7 antibody
    • EXOC1 antibody
    • Exoc7 antibody
    • EXOC7_HUMAN antibody
    • Exocyst complex component 7 antibody
    • Exocyst complex component Exo70 antibody
    • FLJ40965 antibody
    • FLJ46415 antibody
    • KIAA1067 antibody
    • Rexo70 antibody
    • YJL085W antibody
    see all

Images

  • All lanes : Anti-EXOC7 antibody (ab118792) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 83 kDa


    Exposure time : 3 minutes
  • Detection of EXOC7 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118792 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab118792 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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