The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration. PubMed: 21566143
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 21108927
FunctionComponent of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
Sequence similaritiesBelongs to the EXO70 family.
DomainThe N-terminus is involved in SEC8 and ARHQ binding. The C-terminus is required for translocation to the plasma membrane.
Cellular localizationCytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
EXOC7 antibody (ab57402) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
Western blot - EXOC7 antibody (ab57402)
Predicted band size : 83 kDa EXOC7 antibody (ab57402) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-EXOC7 antibody (ab57402)
Overlay histogram showing HeLa cells stained with ab57402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57402, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
References for Anti-EXOC7 antibody (ab57402)
This product has been referenced in:
Hubert T et al. Actin and Arp2/3 localize at the centrosome of interphase cells. Biochem Biophys Res Commun404:153-8 (2011).
Read more (PubMed: 21108927) »
Barkefors I et al. Exocyst Complex Component 3-like 2 (EXOC3L2) Associates with the Exocyst Complex and Mediates Directional Migration of Endothelial Cells. J Biol Chem286:24189-99 (2011).
Read more (PubMed: 21566143) »